期刊论文

Effects of Oolong tea aqueous extract on proliferation and apoptosis of human colon cancer LOVO cells in vitro

中文

福建农林大学学报(自然科学版)

1671-5470

2016

45

6

638-643

10.13323/j.cnki.j.fafu(nat.sci.).2016.06.005

31471590:国家自然科学基金 31471706:国家自然科学基金 CARS-23-C:国家茶叶产业技术体系项目 :教育部***奖励计划项目 IRT0963:创新团队发展计划 2015R1012-3:福建省省属公益类科研院所专项

本校为第一机构

以赛珍珠5800铁观音为材料,测定乌龙茶中水浸出物、茶多酚、游离氨基酸、可溶性糖、黄酮类物质、没食子酸、8种儿茶素和3种生物碱的含量;并将0、0.2、0.4、0.6、0.8和1.0 mg·mL~(-1)乌龙茶水提取物作用于体外培养的人大肠癌LOVO细胞中,分别处理24和48 h后,采用四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率,并在倒置显微镜下观察细胞形态的变化;以0.6和0.8 mg·mL~(-1)乌龙茶水提取物处理LOVO细胞48 h,采用流式细胞仪检测细胞凋亡率,扫描电镜下观察细胞凋亡情况.结果表明,乌龙茶中各化学成分的含量均较高,水浸出物含量达40.8%以上,茶多酚含量14.2%,游离氨基酸含量1.8%,可溶性糖含量6.9%,黄酮类物质...

To investigate active constituents of Oolong tea in inhibiting the proliferation of LOVO cells and inducing apoptosis, Sai zhenzhu 5800 Tieguanyin, a type of Oolong tea, was detected in terms of water extract tea polyphenols, free amino acids, water sol-uble sugar, flavones, gallic acid, 8 catechins and 3 alkaloids. Then the Oolong tea aqueous extract was added to human colon cancer LOVO cells, which were cultured in vitro in concentrations of 0, 0.2, 0.4, 0.6, 0.8, 1.0 mg?mL-1 for 24 h and 48 h, respectively. Cell morphology was observed under inverted microscope, and 3-( 4, 5-dimethylthiazol...