Funding: This research was funded by National Natural Science Foundation of China (grant numbers 91317312, 31570372 and 91417300-03), Scientific Research Fund of Hunan Provincial Education Department (grant number 18K042).
机构署名：
本校为第一且通讯机构
院系归属：
生物科学技术学院
摘要：
Small signaling peptides (SSPs) are a class of short peptides playing critical roles in plant growth and development. SSPs are also involved in the phytohormone signaling pathway. However, identification of mature SSPs is still a technical challenge because of their extremely low concentrations in plant tissue and complicated interference by many other metabolites. Here, we report an optimized protocol to extract SSPs based on protoplast extraction and to analyze SSPs based on tandem mass spectrometry peptidomics. Using plant protoplasts as the...
