期刊论文、会议论文

Yin, Yu-Long

英文

Livestock Science

1871-1413

2007

109

1-3

19-23

10th International Symposium on Digestive Physiology in Pigs

MAY 25-27, 2006

Vejle, DENMARK

Chinese Acad Sci, Inst Subtrop Agr, Changsha 410128, Hunan, Peoples R China.^Cent S Univ, Xiang Ya Med Sch, Mol Biol Res Inst, Changsha 410078, Hunan, Peoples R China.^Hunan Agr Univ, Dept Anim Sci, Changsha 410128, Peoples R China.^Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS

ELSEVIER SCIENCE BV

10.1016/j.livsci.2007.01.027

本校为其他机构

This study was conducted to test the hypothesis that porcine jejunal mucosal cells can degrade all essential amino acids (EAA). Jejunal mucosal cells (primarily enterocytes) were isolated from 50-day-old healthy pigs and incubated at 37 degrees C for 45 min in Krebs buffer containing plasma concentrations of amino acids and one of the following L-[1-C-14]- or L-[U-C-14]-amino acids plus unlabeled tracers at 0.5, 2, or 5 mM: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. There was active transamination of leucine, isoleucine and valine in t...