ACKNOWLEDGMENTS We would like to thank the Research Foundation of Hunan Provincial Education Department of China (13B045), Natural Science Foundation of Hunan Province of China (2015JJ3075), National Undergraduate Training Program for Innovation and Entrepreneurship of China (GSCX1513) and Hunan Provincial Undergraduate Training Program for Innovation and Entrepreneurship of China (SCX1513).
机构署名：
本校为第一且通讯机构
院系归属：
农学院
生物科学技术学院
摘要：
A novel lipolytic enzyme-producing endophytic strain PC2 was successfully isolated from the seeds of an ideal bioenergy plant Pistacia chinensis Bunge. Based on the analysis of morphology and 16S rRNA sequence, bacterial strain PC2 was identified as a subspecies of Pseudomonas putida, therefore named as P. putida PC2. Whole-genome sequencing showed PC2 contained a 1224-nucleotide lipase gene (named lip-PC2) predicted to encode a 407-amino-acid protein. Purified lipases from both the original PC2 strain and heterologously expressed Escherichia c...
