The present study investigated the effect of the replacement of chemical fertilizers by organic fertilizers on agronomic traits of tobacco at maturity as well as on the conventional chemical quality of post-roasted tobacco leaves. To better understand the relationship between tobacco metabolites and roasted tobacco under organic nitrogen replacement treatments, post-roasting tobacco leaves were analyzed by an untargeted metabolomics analytical approach to identify key metabolites applicable to predicting tobacco quality. Methods: Yunyun Tobacco 87 was adopted as the test material in a field pl...
