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A Novel Method in Identifying Pyroptosis and Apoptosis Based on the Double Resonator Piezoelectric Cytometry Technology

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成果类型:
期刊论文
作者:
Li, Wenwei;Li, Jing;Wu, Yanyang;Zhou, Tiean
通讯作者:
Zhou, Tiean(tieanzhou@hunau.edu.cn);Wu, Yanyang(wuyanyang2002@hunau.edu.cn)
作者机构:
[Zhou, Tiean; Li, Wenwei] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
[Zhou, Tiean; Li, Wenwei] Hunan Prov Engn Technol Res Ctr Cell Mech & Funct, Changsha 410128, Peoples R China.
[Li, Jing; Wu, Yanyang] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.
通讯机构:
[Tiean Zhou; Yanyang Wu] A
Authors to whom correspondence should be addressed.<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Engineering Technology Research Center for Cell Mechanics and Function Analysis, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
语种:
英文
关键词:
double resonator piezoelectric cytometry;cell pyroptosis;cell apoptosis;HeLa cell;cell-exerted stress;cell viscoelasticity
期刊:
Biosensors
ISSN:
2079-6374
年:
2023
卷:
13
期:
3
页码:
356-
基金类别:
Conceptualization, W.L., Y.W. and T.Z.; methodology, W.L., Y.W. and T.Z.; software, W.L.; validation, W.L., Y.W. and T.Z.; formal analysis, W.L.; investigation, W.L. and J.L.; resources, W.L., Y.W. and T.Z.; data curation, W.L.; writing—original draft preparation, W.L.; writing—review and editing, T.Z.; visualization, W.L., Y.W. and T.Z.; supervision, W.L., Y.W. and T.Z.; project administration, Y.W. and T.Z.; funding acquisition, Y.W. and T.Z. All authors have read and agreed to the published version of the manuscript. This work was funded by the National Natural Science Foundation of China (31741045), Science and Technology Innovation Leading Plan for Hunan Provincial High-Tech Industry (2020SK2018), the Key Project Supported by Scientific Research Fund of Hunan Provincial Education Department (19A227).
机构署名:
本校为第一机构
院系归属:
食品科学技术学院
生物科学技术学院
摘要:
In this study, a double resonator piezoelectric cytometry (DRPC) technology based on quartz crystal microbalance (QCM) was first employed to identify HeLa cell pyroptosis and apoptosis by monitoring cells’ mechanical properties in a real-time and non-invasive manner. AT and BT cut quartz crystals with the same frequency and surface conditions were used concurrently to quantify the cells-exerted surface stress (ΔS). It is the first time that cells-exerted surface stress (ΔS) and cell viscoelasticity have been monitored simultaneously during p...

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