A protocol was developed for the isolation, culture and plant regeneration of protoplasts isolated from cell suspension cultures of the kiwifruit cultivar 'Qinxiang' (Actinidia deliciosa). Stirring the initiated calli to fine cell aggregates and the supplementing the medium with 0.01% Pluronic F-68 were essential for cell suspension establishment and subculture. A large number of viable protoplasts were obtained from the 4 h enzyme digestion of 2-3 d cell suspension subcultures. Nurse culture was beneficial to A. deliciosa protoplast...
