Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants

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成果类型：

期刊论文

作者：

Jiang, Yingli;Xiao, Zhi;Luo, Zhaopeng;Zhou, Suhuai;Tong, Chaoyun;...

通讯作者：

Wei, PC;Li, J

作者机构：

[Jiang, Yingli; Tong, Chaoyun; Wei, Pengcheng; Zhou, Suhuai; Jin, Shan; Liu, Xiaoshuang; Xiao, Zhi] Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China.

[Xu, Rongfang; Tong, Chaoyun; Jin, Shan; Qin, Ruiying; Li, Juan; Xiao, Zhi] Anhui Acad Agr Sci, Anhui Prov Key Lab Rice Germplasm Innovat & Mol, Hefei 230031, Peoples R China.

[Wei, Pengcheng; Zhou, Suhuai; Xiao, Zhi] Anhui Agr Univ, Adv Acad, Res Ctr Biol Breeding Technol, Hefei 230036, Peoples R China.

[Luo, Zhaopeng] Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China.

[Pan, Lang] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China.

通讯机构：

[Wei, PC ; Li, J ] A

Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China.

Anhui Acad Agr Sci, Anhui Prov Key Lab Rice Germplasm Innovat & Mol, Hefei 230031, Peoples R China.

Anhui Agr Univ, Adv Acad, Res Ctr Biol Breeding Technol, Hefei 230036, Peoples R China.

语种：

英文

关键词：

CRISPR;TadA variants;UNG;base editing;plants

期刊：

Trends in Biotechnology

ISSN：

0167-7799

年：

2025

卷：

期：

页码：

1765-1787

DOI：

10.1016/j.tibtech.2025.03.001

基金类别：

Author contributions Conceptualization, J.L. and P.W.; methodology, Y.J., Z.X., and S.Z.; investigation, Y.J., Z.X., Z.L., C.T., S.J., and X.L.; writing – original draft, Y.J. and J.L.; writing –review and editing, P.W.; funding acquisition, R.Q., J.L., and P.W.; resources, R.Q., R.X., and P.L.; supervision, P.W.

机构署名：

本校为其他机构

院系归属：

植物保护学院

摘要：

Plant cytosine (C)-to-guanine (G) base editors (CGBEs) have been established but suffer from limited editing efficiencies and low outcome purities. This study engineered a cod uracil DNA glycosylase (cod UNG, coUNG) from the cold-adapted fish Gadus morhua for plant CGBE, demonstrating 1.71- to 2.54-fold increases in C-to-G editing efficiency compared with the CGBE using human UNG (hUNG). Further engineering took advantage of TadA-8e-derived cytidine deaminases (TadA-CDs). These variants induced C substitutions with efficiencies ranging from 26.28% to 30.82% in rice cells, whereas adenine (A) c...

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Improving plant C-to-G base editors with a cold-adapted glycosylase and TadA-8e variants

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