期刊论文

Li, Juan;Wei, Pengcheng

英文

Trends in Biotechnology

0167-7799

2025

10.1016/j.tibtech.2025.03.001

Author contributions Conceptualization, J.L. and P.W.; methodology, Y.J., Z.X., and S.Z.; investigation, Y.J., Z.X., Z.L., C.T., S.J., and X.L.; writing – original draft, Y.J. and J.L.; writing –review and editing, P.W.; funding acquisition, R.Q., J.L., and P.W.; resources, R.Q., R.X., and P.L.; supervision, P.W.

本校为其他机构

Plant cytosine (C)-to-guanine (G) base editors (CGBEs) have been established but suffer from limited editing efficiencies and low outcome purities. This study engineered a cod uracil DNA glycosylase (cod UNG, coUNG) from the cold-adapted fish Gadus morhua for plant CGBE, demonstrating 1.71- to 2.54-fold increases in C-to-G editing efficiency compared with the CGBE using human UNG (hUNG). Further engineering took advantage of TadA-8e-derived cytidine deaminases (TadA-CDs). These variants induced C substitutions with efficiencies ranging from 26.28% to 30.82% in rice cells, whereas adenine (A) c...