期刊论文

Zeng, F. -H.

英文

BIOLOGIA PLANTARUM

0006-3134

2010

54

1

131-134

10.1007/s10535-010-0020-4

Received 5 April 2008, accepted 1 December 2008. Abbreviations: BA - 6-benzyladenine; GA3 - gibberellic acid; IAA - indole-3-acetic acid; IBA - indole-3-butyric acid; MS - Murashige and Skoog; NAA - naphthalene acetic acid; PBU - N-phenyl-N’-[6-(2-chlorobenzothiazol)-yl] urea; TDZ - 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron); 2,4-D - 2,4-dichlorophenoxyacetic acid. Acknowledgements: The research was supported by the Natural Science Foundation (5011730) and Science and Technology Tackle Key Problem (2006B20101011) of Guangdong province. * Corresponding author; e-mail: zengfuhua@gmail.com

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Seedling hypocotyls were used as explants to establish a regeneration protocol for Eucalyptus urophylla and N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl] urea (PBU), one kind of di-substituted urea, was found useful growth regulator. The hypocotyls incubated on a modified Murashige and Skoog medium (SPCa), supplemented with 6.6 μM PBU and 0.57 μM indole-3-acetic acid (IAA) dedifferentiated and form calli (100% after 7 d). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained their darkening. In addi...