The optimization of ABA determination conditions of an amperometric immunosensor based on gold nanoparticle（nano-Au）/thionine modified gold electrode for the determination of abscisic acid（ABA）was researched. The sensor was prepared by the principle of the oxidation reaction of thionine by H2O2 which catalyzed by horseradish peroxidase （HRP）,and thionine and ABA antibody labeled with HRP was adsorbed on electrode surface by covalence. Bovine serum albumin （BSA）was employed to block the possible remaining active sites of the thionine mon...
