期刊论文

中文

生物技术通报

1002-5464

2008

2

136-139

10.13560/j.cnki.biotech.bull.1985.2008.02.044

南华大学硕士启动项目（5-03-XJQ-03-016）

本校为其他机构

分别以特早50、青梗菜心、9872和四九菜心(19)为供试材料,进行了基因型、温度、碳源等因素对菜心花药培养诱导胚状体影响的研究.结果 表明:不同基因型菜薹花药诱导率的差异显著,在改良B5固体培养基上特早50、9872、四九菜心(19)的诱导率依次降低,而青梗菜心诱导不出胚状体;不同温度的前处理对9872花药的诱导率也存在影响,其中以4℃低温1预处理幼花序1～2d和33℃(2温预培养24～48h的处理效果最佳;碳源对胚状体的诱导也有较大影响,其中蔗糖效果好于麦芽糖,且质量浓度为13%的蔗糖对特早50诱导率最高,而13%的麦芽糖诱导不出胚状体.

The main factors of stalk anther culture such as genotype,low temperature of buds,high temperature culture and carbon source were studied. The results show that genotype plays a decisive role in anther ctdture,the induction frequency of embroids among different plants differed significantly,low temperature of buds can obviously improve the induction frequency of embroids;and 1-2 days high temperature at the beginning culture were beneficial to the successful development of cultured microspore s into cmbryos. The carbon source was also influenced on t...