期刊论文

Research Advances in Gene Editing and Plant-virus Interaction and Their Application in Breeding Virus-resistant Crops

中文

生物技术通报

1002-5464

2018

34

8

8-16

10.13560/j.cnki.biotech.bull.1985.2018-0462

国家自然科学基金项目（91440103）；

本校为第一机构

病毒病害是危害农作物生长的重要病害,最经济有效的防治策略是选育对病毒有抗性的品种。随着病毒与植物互作研究的深入,我们对于病毒在其生活周期中与其所依赖的植物基因的互作有了深入的认识。病毒进入植物细胞后,其病毒颗粒的解聚、基因的表达、基因组的复制、其核酸蛋白复合体通过包间连丝和韧皮部的移动等过程都依赖病毒基因与植物特定基因的相互作用。这些植物基因的隐性突变可以导致植物对病毒的抗性。这类变异存在于作物的自然变异群体中,可以直接应用于抗病育种。CRISPR/Cas介导的基因编辑技术是一种可以在动植物体内对DNA序列进行定点突变的新技术,在过去几年里得到了突飞猛进的发展。...

Viral diseases impose serious threat to crop production and the best management strategy is to breed virus-resistant crop varieties for production. Research on plant-virus interaction has revealed mechanistic insights on the interactions between viral and plant genes which are essential for viruses to complete their life cycle. Once a virus enters its host plant cells, its particle disassembly, gone expression, genome replication, and rihonucleoprotein complex movement through plasmodesmata and phloem tissue are all dependent on interactions between viral products and specific plant genes. The...