期刊论文

中文

生物技术通报

1002-5464

2020

36

12

64-74

10.13560/j.cnki.biotech.bull.1985.2020-1252

国家自然科学基金项目（31672057）；

本校为第一机构

本实验研究了温室条件下,连续两年施用异噁草酮后土壤微生物群落组成及N转化功能微生物的响应。第二次施药后第7天、15天、30天、60天和90天采集土壤,测定异噁草酮残留量和相关微生物指标。研究结果显示异噁草酮降解半衰期为17.4 d。施药后第15天细菌拷贝数降低,Alpha多样性增加;真菌拷贝数在第15天和60天显著降低,Alpha多样性在第7天和第90天显著降低。主成分分析(PCoA)显示,整个培养期间细菌和真菌的群落组成显著改变。分子生态网络分析表明,异噁草酮增加分子生态网络中节点数、连接数、平均度和平均路径长度,显著改变模块枢纽与联络者。FAPROTAX功能预测结果表明异噁草酮主要降低反硝化作用。...

This work aims to investigate the responses of soil microbial communities and microbes with nitrogen-transforming functions to 2 years consecutive application of clomazone under greenhouse conditions.Soil was sampled after 7,15,30,60,and 90 d of second application and clomazone residue concentration and relevant microbial parameters were determined.The half-lives of clomazone in this experiment were 17.4 d.The results showed that copy numbers of bacteria decreased,whereas bacterial Alpha diversity increased.Copy numbers of fungi(day 15 and day 60)and fungal diversity(day 7 and day 90)significa...