Introduction This study aims to investigate the factors influencing ecosystem service trade-offs/synergies (TOSs) in major agricultural production areas in the southern hilly region and to propose optimization strategies to promote ecosystem sustainability in agricultural areas.Methods The study used a geographical detector to analyze the determinants influencing the ecosystem service trade-offs as well as correlation analysis, geographically weighted regression (GWR), and a geographical detector to analyze the spatial and temporal evolution of ecosystem service and TOS relationships from 2000...