The amplified fragment lenghth polymorphism(AFLP) is based on polymerase chain reaction(PCR) technique, and amplify with restricted genetic fragment, which involved in restriction of DNA and ligation of the adaptor, and selective amplification and gel analysis. We briefly summarized the application of AFLP in aquatic animals, such as the genetic multiplicity, related determination, generating genetic maps and useful breeding. The application perspective of AFLP in developme...