MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing

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成果类型：

期刊论文

作者：

Huang, Qi-Xing*;Cheng, Xin-Yue;Mao, Zhen-Chuan;Wang, Yun-Sheng;Zhao, Li-Lin;...

通讯作者：

Huang, Qi-Xing

作者机构：

[Yan, Xia; Cheng, Xin-Yue; Huang, Qi-Xing; Xu, Ru-Mei] Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China.

[Xie, Bing-Yan; Mao, Zhen-Chuan; Huang, Qi-Xing; Wang, Yun-Sheng] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing, Peoples R China.

[Wang, Yun-Sheng] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha, Hunan, Peoples R China.

[Zhao, Li-Lin] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China.

[Ferris, Virginia R.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA.

通讯机构：

[Huang, Qi-Xing] B

Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China.

语种：

英文

关键词：

MicroRNAs;RNA sequencing;cDNA libraries;Expressed sequence tags;RNA stem-loop structure;Caenorhabditis elegans;Heat shock response;Gene expression

期刊：

PLOS ONE

ISSN：

1932-6203

年：

2010

卷：

期：

页码：

e13271

DOI：

10.1371/journal.pone.0013271

基金类别：

National Basic Research and Development ProgramNational Basic Research Program of China [2009CB119200]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30570288]

机构署名：

本校为其他机构

院系归属：

园艺园林学院

摘要：

Background: MicroRNAs (miRNAs) are considered to be very important in regulating the growth, development, behavior and stress response in animals and plants in post-transcriptional gene regulation. Pinewood nematode, Bursaphelenchus xylophilus, is an important invasive plant parasitic nematode in Asia. To have a comprehensive knowledge about miRNAs of the nematode is necessary for further in-depth study on roles of miRNAs in the ecological adaptation of the invasive species. Methods and Findings: Five small RNA libraries were constructed and se...

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MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing

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