Studies on microbial ammonium removal have focused on the heterotrophic nitrification of microorganisms and have rarely studied the role of ammonium assimilation. In this study, Rhodococcus erythropolis strain Y10 with the capacity of aerobic denitrification was screened from the surface flow constructed wetlands that treat high-strength ammonium swine wastewater. Instead of through nitrification, this strain removed ammonium through heterotrophic ammonium assimilation, with the removal rate of 9.69 mg/L/h. The KEGG nitrogen metabolism pathway ...