期刊论文

中文

分子细胞生物学报

1673-520X

2008

41

4

255-264

10.3321/j.issn:1673-520X.2008.04.002

本校为第一机构

对苎麻属4个种的rbcL序列进行了PCR扩增和T/A克隆产物的序列测定,扩增出长度为1370bp的rbcL序列。从GenBank中选取荨麻科8个属的rbcL序列,将所有物种的rbcL序列排序后,总长度约为1428bp。其中保守位点1263个,变异位点165个,信息位点71个。利用MEGA3.1软件构建荨麻科植物的MP和NJ系统树,并根据rbcL区碱基序列的差异计算种间的遗传距离,从而进一步探讨荨麻科植物之间的亲缘关系。其中,赤车属、锥头麻属、藤麻属、楼梯草属和冷水花属聚为一支,具有较近的亲缘关系;墙草属、单蕊麻属和苎麻属聚为另一组;而荨麻属植物在一级分支中就与荨麻科其他属植物分开,说明该属与其他属的亲缘关系较远。

The sequences of rbcL from four species of Boehmeria were analyzed in this study by PCR amplification and directly sequencing. The sequences of PCR products were about 1,370 bp. RbcL sequences of nine generas were chosen from GenBank, then the rbcL of 13 species were sequenced with clustal X1.83. After sequencing, the length of rbcL was 1,428 bp and has 1,263 conserved sites, 165 variable sites and 71 information sites. By using MEGA3.1, MP tree and NJ tree of Urticaceae were constructed. Based on the difference in rbcL squence, the genetic distances among species were calculated. With these i...