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Genome-wide identification, localization, and expression analysis of proanthocyanidin-associated genes in Brassica

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成果类型:
期刊论文
作者:
Liu, Xianjun;Lu, Ying;Yan, Mingli;Sun, Donghong;Hu, Xuefang;...
通讯作者:
Liu, Zhongsong
作者机构:
[Guan, Chunyun; Lu, Ying; Liu, Zhongsong; Liu, Xianjun; Sun, Donghong; Liu, Shuyan; Chen, Sheyuan] Hunan Agr Univ, Oilseed Crops Inst, Changsha, Hunan, Peoples R China.
[Liu, Xianjun] Coll Life Sci Resources & Environm Sci, Yichun, Peoples R China.
[Yan, Mingli] Hunan Univ Sci & Technol, Sch Biol, Xiangtan, Peoples R China.
通讯机构:
[Liu, Zhongsong] H
Hunan Agr Univ, Oilseed Crops Inst, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
BAC library;Brassica spp.;gene cloning;proanthocyanidin biosynthesis;seed color
期刊:
FRONTIERS IN PLANT SCIENCE
ISSN:
1664-462X
年:
2016
卷:
7
期:
DECEMBER2016
页码:
1831
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31101176, 31271762]
机构署名:
本校为第一且通讯机构
院系归属:
农学院
摘要:
Proanthocyanidins (PA) is a type of prominent flavonoid compound deposited in seed coats which controls the pigmentation in all Brassica species. Annotation of Brassica juncea genome survey sequences showed 72 PA genes; however, a functional description of these genes, especially howtheir interactions regulate seed pigmentation, remains elusive. In the present study, we designed 19 primer pairs to screen a bacterial artificial chromosome (BAC) library of B. juncea. A total of 284 BACclones were identified and sequenced. Alignment of the sequenc...

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