Reference Gene Selection for qRT-PCR Analysis in the Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Li, Rumei;Xie, Wen;Wang, Shaoli;Wu, Qingjun;Yang, Nina;...

通讯作者：

Zhou, Xuguo

作者机构：

[Li, Rumei; Zhou, Xiaomao; Bai, Lianyang] Hunan Agr Univ, Inst Pesticide, Changsha, Hunan, Peoples R China.

[Yang, Xin; Xie, Wen; Zhang, Youjun; Wang, Shaoli; Yang, Nina; Wu, Qingjun; Xu, Baoyun; Li, Rumei; Pan, Huipeng] Chinese Acad Agr Sci, Dept Plant Protect, Inst Vegetables & Flowers, Beijing 100193, Peoples R China.

[Zhou, Xuguo; Pan, Huipeng] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.

通讯机构：

[Zhou, Xuguo] U

Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.

语种：

英文

关键词：

Gene expression;Adenosine triphosphatase;Myosins;Polymerase chain reaction;Insecticides;Tomatoes;Heat shock response;Plants

期刊：

PLOS ONE

ISSN：

1932-6203

年：

2013

卷：

期：

页码：

e53006-

DOI：

10.1371/journal.pone.0053006

基金类别：

National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [31025020]; 973 ProgramNational Basic Research Program of China [2013CB127602]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30900153]

机构署名：

本校为第一机构

院系归属：

植物保护学院

摘要：

BACKGROUND: Accurate evaluation of gene expression requires normalization relative to the expression of reliable reference genes. Expression levels of "classical" reference genes can differ, however, across experimental conditions. Although quantitative real-time PCR (qRT-PCR) has been used extensively to decipher gene function in the sweetpotato whitefly Bemisia tabaci, a world-wide pest in many agricultural systems, the stability of its reference genes has rarely been validated. RESULTS: In this study, 15 candidate reference genes from B. tabaci were evaluated using two Excel-based algorithm...

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Reference Gene Selection for qRT-PCR Analysis in the Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

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