期刊论文

Wood, AJ

英文

BIOLOGIA PLANTARUM

0006-3134

2004

48

4

531-535

10.1023/B:BIOP.0000047148.20309.88

Received 30 December 2002, accepted 20 June 2003. Abbreviations: NiA - nicotinic acid; NNMT - S-adenosyl-L-methionine nicotinic acid-N-methyltransferase; PAGE - polyacrylamide gel electrophoresis; PPFD - photosynthetic photon flux density; SAM - S-adenosyl-L-methionine; TRG - trigonelline. Acknowledgments: This work was supported in part by grants to AJW from the Illinois Soybean Program Operating Board (98-ISPOB-24-212-3) and Illinois Council on Food and Agricultural Research (project number 00E-079-3). ** Corresponding author; fax: (+01) 618 4533441, e-mail: wood@plant.siu.edu.

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Trigonelline (TRG), which act as a cell cycle regulator and a compatible solute in response to salinity and water-stress, is the N-methyl conjugate of nicotinic acid the formation of which is catalyzed by S-adenosyl-L-methionine nicotinic acid-N-methyltransferase. The enzyme was purified 2650-fold from soybean (Glycine max L.) leaves with a recovery of 4 %. The purification procedure included ammonium sulfate (45 - 60 %) precipitation, linear gradient DEAE-Sepharose chromatography, adenosine-agarose affinity chromatography, hydroxyapatite chrom...