Purification, characterization and enzymatic synthesis of theaflavins of polyphenol oxidase isozymes from tea leaf (Camellia sinensis)

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成果类型：

期刊论文

作者：

Teng, Jie;Gong, Zhihua;Deng, Yanli;Chen, Ling;Li, Qin;...

通讯作者：

Xiao, Wenjun

作者机构：

[Gong, Zhihua; Shao, Yuanyuan; Li, Qin; Xiao, Wenjun; Lin, Ling; Deng, Yanli; Chen, Ling; Teng, Jie] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.

[Gong, Zhihua; Shao, Yuanyuan; Li, Qin; Xiao, Wenjun; Lin, Ling; Deng, Yanli; Teng, Jie] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.

[Li, Qin; Xiao, Wenjun] Hunan Agr Univ, Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410128, Hunan, Peoples R China.

[Teng, Jie] South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China.

通讯机构：

[Xiao, Wenjun] H

Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

Alcohols;Amino acids;Chromatography;Enzymes;Flavonoids;Isoenzymes;Phenols;Purification;Substrates;Catechins;Enzymatic synthesis;Enzyme substrates;Epigallocatechin gallate;Manufacturing conditions;Optimal temperature;Polyphenol oxidase;Theaflavins;Tea

期刊：

LWT

ISSN：

0023-6438

年：

2017

卷：

页码：

263-270

DOI：

10.1016/j.lwt.2017.05.065

基金类别：

This work was supported by the National Hunan Provincial Science and Technology Support Program (Grant No. 2012WK2009) and the Hunan Education Department Science Research Program (Grant No. 13A034).

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

Polyphenol oxidase (PPO) plays a key role in tea processing, converting catechins into theaflavines. Two PPOs were isolated and purified from tea (Camellia sinensis) leaves. PPO1 and PPO2 exhibited molecular weights of 85 KDa and 42 KDa, respectively. PPO activities were evaluated at different temperatures and pH values to determine thermal stability as well as the optimal temperature and pH. Moreover, PPO1 and PPO2 were allowed to react with catechol, epigallocatechin (EGC), epigallocatechin gallate (EGCG), L-tyrosine, guaiacol, and pyrogallic...

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Purification, characterization and enzymatic synthesis of theaflavins of polyphenol oxidase isozymes from tea leaf (Camellia sinensis)

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