The invention provides a rapid inoculation and identification method for a citrus bacterial canker disease. The method comprises the following steps of (1) sample leaf collection; (2) pathogen cultureof a citrus bacterial canker disease; (3) identification of the canker disease inoculated into sample leaves; (4) rooting culture of the sample leaves; and (5) culture management of the sample leaves. Aiming at the disadvantages and shortcomings of existing resistance identification of the citrus bacterial canker disease, culture and identification modes are innovated through in vitro culture ofth...