期刊论文

中文

实验技术与管理

1002-4956

2007

24

5

29-31

10.3969/j.issn.1002-4956.2007.05.010

本校为第一机构

以三叶浮萍（Lemma Paucicostata）为试验材料,研究了3种提取方法及6种不同提取液对浮萍体内叶绿素提取效果的影响,并测定了叶绿素在6种不同提取液中的稳定性。结果表明:6种提取液的吸收光谱基本相似,可以用Arnon法的计算公式来计算叶绿素的含量。3种提取方法中,直接浸取法对叶绿素的提取效果最好,但速度最慢;而研磨法在提取过程叶绿素损失较大;将样品冷冻后用50℃提取液提取方法的提取速度最快且操作简便,是最佳的叶绿素提取方法。试验采用的6种提取液除无水乙醇外,叶绿素在其他5种提取液中较稳定,不易见光分解。

Taking Lemma Paucicostata as the object, the influence of 3 different methods and 6 different extracts on the ectrating efficiencies was studied. The results showed that the absorption spectrums of 5 extracts were identical with that of acetone solution, so Arnon＇formula of chlorophyll was suitable for the other extracts. Soaking methods could extract the most chlorophyll, but it was very slowly; and chlorophyll was lost so much by Arnon methods. The best method was that using 50 ℃ extracts soaking the freezing sample because it was the faste...