期刊论文

Isolation,Identification and Growth Characteristics of Main Pathogens of Kiwifruit Soft Rot

中文

核农学报

1000-8551

2020

34

11

2425-2434

10.11869/j.issn.100-8551.2020.11.2425

2017NK2071:湖南省科技厅重点项目 2018NK2013:湖南省科技厅重点项目

本校为第一机构

为探究猕猴桃果实采后软腐病害的主要病原菌及其生长特性,2017年9月于湖南凤凰县猕猴桃基地采集具有软腐病症状的100个果实病样,取患病猕猴桃病健交界处果肉,进行病原菌的分离纯化、菌株形态学观察、病原菌的分子生物学鉴定试验,同时分析不同培养基、碳源、氮源、温度和pH值对病原菌生长的影响。结果表明,菌种鉴定为葡萄座腔菌( Botryosphaeria dothidea)和间座壳菌(拟茎点霉菌有性态,Diaporthe phaseolorum) 。两种菌在PSA、YPGA、PDA、SDA培养基的生长速度均较快,葡萄座腔菌和间座壳菌生长速率分别在16.1 ~ 18.6 mm·d~(-1)和13.9 ~ 16.5 mm·d~(-1)之间,且菌丝密度均较稠密。淀粉、蔗糖和麦芽...

To explore the main pathogens and its’ growth characteristics of postharvest soft rot of kiwifruit, In September 2017, 100 fruit samples with soft rot symptoms were collected at the kiwifruit base in Fenghuang County, Hunan Province. The flesh between the diseased and the good were taken out to isolate and purify the pathogens. Then the morphologies of strains were observed, and molecular biological of pathogens were identified. At the same time, the effects of media, carbon source, nitrogen source, temperature, and pH value on the growth of p...