期刊论文

Identification of Polyphenolic Compounds in Daylily and Influence of Blanching and Storage on Their Stability

中文

核农学报

1000-8551

2021

35

12

2787-2798

10.11869/j.issn.100-8551.2021.12.2787

湖南省自然科学基金面上项目（2020JJ4367）；

本校为第一机构

为鉴定黄花菜多酚类化合物的结构并研究漂烫与贮藏对其稳定性的影响,本研究采用响应面和正交试验设计,四极杆飞行时间液质串联系统,以及三重四极杆液质串联系统,研究了超声波辅助提取黄花菜多酚,HPD-600大孔树脂吸附多酚,乙醇解吸附多酚的最佳条件,对多酚类化合物进行分离纯化和结构鉴定,并对新鲜、漂烫与室温贮藏的黄花菜多酚类化合物含量进行分析。结果表明,超声辅助提取黄花菜多酚类化合物的最佳条件为:乙醇浓度66%,时间22 min,温度43℃,料液比1 ∶12 g·mL~(-1),此条件下多酚得率为2.12%; HPD-600大孔树脂吸附多酚的最佳条件为温度50℃,pH值6,吸附时间3 h,此条件下多酚吸附率为28.76%;无水乙...

In order to identify the polyphenolic compounds in daylily and study the influence of blanching and storage at room temperature on their stability,response surface methodology and orthogonal experiment design,quadrupole time-offlight liquid mass tandem system and triple quadrupole liquid mass tandem system were employed to optimize the technological parameters for extraction and identification of polyphenols by ultrasound-assisted extraction,and adsorption by HPD-600 macroporous resin,and desorption by ethanol. The content of polyphenols from f...