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拟南芥AtGSTZ1基因启动子与GUS重组载体的构建及转化#@#@#Construction and Transformation of Recombinant Vector Consisting of GUS Gene and Zeta Class Glutathione S-Transferase 1 Promoter in Arabidopsis thaliana

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成果类型:
期刊论文
作者:
蒋嘉彦;董文;黄崇瑞;彭怡;黄丽华;...
作者机构:
湖南农业大学生物科学技术学院,湖南 长沙
湖南农业大学国际学院,湖南 长沙
语种:
中文
关键词:
谷胱甘肽转移酶;AtGSTZ1基因;启动子
关键词(英文):
GUS;Glutathione S-Transferases;AtGSTZ1 Gene;Promoter;GUS
期刊:
植物学研究
ISSN:
2168-5665
年:
2019
卷:
08
期:
2
页码:
158-164
基金类别:
本论文由湖南农业大学创新性实验计划项目XCX17066和XCX18102支助。
机构署名:
本校为第一机构
院系归属:
生物科学技术学院
国际学院
摘要:
Abstract: Based on the homology comparison, glutathione transferases (GSTs) in plants are distributed into different classes, which are known to play different roles in plant growth and development. Among GSTs, the function of zeta class GSTs (GSTZ) is not well characterized. Here, the promoter sequence of AtGSTZ1 gene was isolated from Arabidopsis, and a fusion expression vector of AtGSTZ1 promoter and GUS gene was constructed and introduced into Arabidopsis by A. tumefaciens-mediated transformation. Histochemical staining of Arabidopsis indic...
摘要(英文):
Based on the homology comparison,glutathione transferases(GSTs)in plants are distributed into different classes,which are known to play different roles in plant growth and development.Among GSTs,the function of zeta class GSTs(GSTZ)is not well characterized.Here,the promoter sequence of AtGSTZ1 gene was isolated from Arabidopsis,and a fusion expression vector of AtGSTZ1 promoter and GUS gene was constructed and introduced into Arabidopsis by A.tumefaciens-mediated transformation.Histochemical staining of Arabidopsis indicated the AtGSTZ1 promoter drove the GUS expression in Arabidopsis.The res...

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