期刊论文

Preparation of glucose oxidase nanoparticles and their antimicrobial effects in vitro and in vivo

中文

畜牧与兽医

0529-5130

2022

54

02

72-77

国家自然科学基金（21905069）；

本校为第一机构

为了使葡萄糖氧化酶更加稳定,利用可逆加成-断裂链转移自由基聚合反应对葡萄糖氧化酶进行纳米包裹。动态激光散射仪测定显示,包裹后粒径为（12.4±1.0）nm, Zeta电位为（+4.2±0.5）mV。通过冻融循环、长期储存和胰蛋白酶试验证明了包裹后的稳定性。在体外,对大肠杆菌的最低抗菌浓度为0.01 mg/mL。对新西兰大白兔细菌肿包治疗显示,包裹后的葡萄糖氧化酶较包裹前有较好的稳定性和抑菌效果,可作为新型抗菌制剂。

In order to make glucose oxidase more stable, glucose oxidase was nano-coated by reversible addition-fragmentation chain transfer reaction. The particle size after coating was(12.4±1.0)nm and the Zeta potential was(+4.2±0.5)mV, as determined by dynamic laser scattering. The stability of glucose oxidase after coating was demonstrated by freeze-thaw cycles, long-term storage and trypsin tests. In vitro, the lowest antibacterial concentration of the coated glucose oxidase against E.coli was 0.01 mg/mL. The treatment of New Zealand rabbits with b...