期刊论文

中文

畜牧与兽医

0529-5130

2024

56

04

54-60

31902294:国家自然科学基金

本校为其他机构

旨在探明雌、雄刻点血蜱中肠菌群结构特征及其差异.从新疆沙湾市绵羊体表采集刻点血蜱雌、雄蜱,无菌条件下收集雌、雄蜱中肠内容物,提取各样本细菌总DNA,PCR扩增细菌 16S rDNA V3-V4 区,运用Illiumina NovaSeq高通量双末端测序,对数据进行拼接和过滤,扩增子序列变异(ASVs)降噪与物种注释后,对比分析雌、雄蜱中肠菌群结构的组成及差异.结果:雌蜱中肠菌群多样性高于雄蜱;在门水平上,变形菌门、厚壁菌门、异常球菌门、拟杆菌门、放线菌门在雌、雄蜱中肠内相对丰度较高,其中变形菌门相对丰度最高,为 2 个样本的优势菌门,且变形菌门在雄蜱中肠内的含量大于雌蜱;在属水平上,Schlegelella、不动杆菌属、...

In this study,we aimed to investigate the characteristics of and differences in the midgut microbial community structure of female and male Haemaphysalis punctata.The female and male H.punctata were collected from the body of sheep in Shawan,Xinjiang.The midgut contents of both sexes were collected under sterile conditions,the total bacterial DNA of each sample was extracted,and the bacterial 16S rDNA V3-V4 region was amplified by PCR and sequenced using Illiumina NovaSeq high-throughput double-end sequencing.After splicing and filtering the data,denoising with ASVs,and species annotation,the ...