Plants absorb nitrogen, an essential element for their growth and development, mainly in the form of nitrate or ammonium. These nitrogen species have different effects on crops, little is known about their precise influence at both physiological and proteomic levels. This study investigated these mechanisms, hydroponically grown tobacco seedlings treated with three different ratios of NO3- to NH4+ (10:0, 5:5 or 0:10) for 15 days. The results showed that exposure to high NH4+ concentration affected root morphology and inhibited root development....