Results of studies on separation and determination of caffeine,theobr-omine and theophylline with inverse phase HPLC were reported in thispaper.The experiment of HPLC isolated condition was carried out.Effectsof the concentrations of dimethylformaimide,merhanol,acatate and theratios of them to water on the chromatographic behavors of the above purinebases were investigated.The method introduced in this paper possessed theproperty of good separation and repeatability,simple operation and accurateresult.The determinable minimum limits of caffeine...
