期刊论文

中文

茶叶科学

1000-369X

2006

26

4

285-290

10.3969/j.issn.1000-369X.2006.04.010

03JZY3016:湖南省科技厅科技项目 2004BA542C:科技部科技攻关计划

本校为第一机构

利用提取粗酶液在单因素试验和正交试验进行合成茶黄素以及在此基础上利用捣碎茶鲜叶(不提取粗酶液)直接进行合成茶黄素试验以确定最优反应条件.结果表明,在本试验反应体系中,茶黄素酶促合成的最佳条件(茶黄素总量)为:温度为30℃,反应体系的最佳pH值为4.8,底物浓度为0.9 g/100 ml,酶源物浓度为28 g/100 ml(以鲜叶重量计),通氧量为0.4 L/min,最佳反应终止时间是40 min.其中温度和pH值是反应体系中两个极其重要的影响因素(P＜0.05);通氧量也是影响茶黄素酶性合成的必不可少的条件之一.

Obtain the optimum reaction condition of synthesizing theaflavins by enzymatic oxidation, synthesizing theaflavins was studied by raw ppo extracted from fresh tea leaves under single factor and orthogonal experimental design. Based on these results, synthesizing theaflavins was also studied by using breaking fresh tea leaves. The result showed that the optimum condition of theaflavins synthesizing is 30℃ temperature is, under this reaction system, pH value of the present system is 4.8, the concentration of substrates is 0.9 g/100 ml, the conce...