期刊论文

中文

茶叶科学

1000-369X

2006

26

4

280-284

10.3969/j.issn.1000-369X.2006.04.009

(2004BA542C):科技部科技攻关项目

本校为第一机构

建立了配体交换色谱手性流动相法拆分茶氨酸对映体的方法.采用Polaris C_(18)柱;流动相为L-脯氨酸:Cu~(2+)(摩尔比)为2:1,Cu~(2+)浓度为0.5 mmol/L,pH6.8,甲醇的加入体积比为2%;波长254 nm;流速0.9 ml/min;柱温30℃.L-茶氨酸的进样量在0.09542 μg～4.241 μg范围内峰面积与进样量之间线性关系良好,回收率在97.45%～100.4%之间;D-茶氨酸的进样量在0.08486 μg～4.243 μg范围峰面积与进样量之间线性关系良好,回收率在97.07%～100.1%之间.该方法通用,准确.

Ligand exchange chromatography with chiral mobile phases was established for the separation of theanine enantiomers.The optimum condition was ：Polaris C18 column, mobile phase： L-proline： Cu^2＋=2：1, the concentration of Cu^2＋ was 0.5 mmol/L, pH6.8, the volume of methanol is 2%, wavelength： 254 nm;flow rate：0.9 ml/min, column temperature：30℃. There was a good linearity between peak areas and injection quantities of L-Theanine ranging from 0.09542 μg-4.241 μg and D-Theanine ranging from 0.08486 μg-4.243μg. The recovery of L-Theanine range fr...