期刊论文

中文

茶叶科学

1000-369X

2006

26

4

249-252,258

10.3969/j.issn.1000-369X.2006.04.003

广东省农科院资助项目（SR0273）；

本校为第一机构

本研究采用AFLP技术,选用多态性高分辨能力强的5对引物对25个广东茶树群体品种和5个对照品种进行选择性扩增,获得了较清晰的DNA指纹图谱.5对引物共扩增出365条带,平均每对引物扩增73条带,多态性条带338条,占总带数的92.6%.扩增片段大小在50～600 bp之间.采用DPS 2000软件进行聚类,30份供试材料以清凉山茶和清远笔架茶遗传距离最近(0.12752),桂北大叶种和凤凰水仙之间的遗传距离最远(0.5),本实验结果表明广东地方茶树群体品种遗传多样性比较丰富.

Based on AFLP method, AFLP figure were obtained in Guangdong tea plants. In this study, totally 365 bands were amplified by 5 pairs of primes and 73 bands were amplified by each primer pair on average, a total of 338 （92.6%） polymorphic AFLP markers were detected. The results indicated that the genetic distance （GD） based on AFLP data by using DPS 2000 was significant. The GD（0.5） is highest between Guibeidaye and Fenghuangshuixian, but the GD（0.12752） between Qingliangshan tea and Qingyuanbijia tea is low. This indica...