期刊论文

中文

茶叶科学

1000-369X

2010

30

5

350-354

10.3969/j.issn.1000-369X.2010.05.004

:湖南省重大科技专项(茯茶产业化关键技术研究 :示范2008FJ1005)

本校为第一机构

从湖南益阳茶厂2008年生产的金湘益砖茶中分离纯化得到一株"金花"菌,接种至2008年一品茯茶的原料上,控制环境条件让它"发花"。从发花散茶中分离到一类"金花"菌,经过继代纯化,选取一株长势较好的"金花"菌作为供试菌株。在光学显微镜下观察菌株的有性型和无性型形态,并在扫描电镜下观察菌株的子囊孢子（有性阶段）和分子孢子（无性阶段）,同时结合DNA测序,在分子水平上对分离菌株进行鉴定。根据菌株的表观形态、光学显微镜、扫描电镜和DNA序列分析,确认该菌株为冠突散囊菌（Eurotium cristatum）。

A fungi called "Jinhua" that separated from Jing Xiangyi brick tea was produced by Hunan Yiyang Tea Factory in 2008. Inoculating the fungi to the raw material of Yiping Fuzhuan tea of 2008 with "Fungus growing" by controlling the conditions of environment. A strain of "Jinhua" fungi was isolated from the loose tea with fungus growing as a tested fungi through subculture purification. The epigamous and phorozoon of the tested fungi was observed with mi...