期刊论文

中文

茶叶科学

1000-369X

2010

30

6

435-439

10.3969/j.issn.1000-369X.2010.06.005

:湖南省重大科技专项"茯茶产业化关键技术研究 (2008FJ1005):示范" (NYHYZX07-021-12):"十一五"农业部行业支撑计划"名优绿茶高效栽培加工关键技术研究"

本校为第一机构

建立了高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定茶叶中单糖和双糖的检测方法.采用ShodexNH2P-50-4E(4.6mm×250mm,5μm)色谱柱,以水和乙腈为流动相进行梯度洗脱,梯度洗脱程序为:0~14min,85%乙腈;14~16min,85%~70%乙腈;16~28min,70%乙腈;28~32min,70%~85%乙腈,流速:1.0mL/min,柱温:20℃,雾化管温度为30℃,漂移管温度为70℃,氮气压力为310.275Pa.鼠李糖,阿拉伯糖,木糖,果糖,甘露糖,葡萄糖,蔗糖,麦芽糖在3.202~184.828μg范围内程良好的线性关系,8种糖的加标回收率在90.97%~105.07%之间,最低检测限(S/N=3)范围在6.976~1376.297ng.该方法具有操作简便,分离效果好等特点

A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH2P-50-4E(4.6 mm×250 mm,5μm) column was used at 20℃,acetonitrile and water used as mobile phase,for gradient elusion with the flow rate of 1.0 mL/min.The ratio of organic solvent was controlled by a gradient elution procedure.The procedure was as follows:85%acetonitrile in 0～14 min,70%～80%acetonitrile 14～16 min,70%acetonitrile 16～28 min,then 70%～80%acetoni...