期刊论文

Functional Analysis of Glutathione Peroxidase Encoding Gene CsGPX1 in Camellia sinensis

中文

茶叶科学

1000-369X

2019

39

4

382-391

10.3969/j.issn.1000-369X.2019.04.003

国家自然科学基金(31670689) 湖南省重点研发计划(2018NK2032).

本校为第一机构

为明确茶树[Camellia sinensis (L.) O. Kuntze.]谷胱甘肽过氧化物酶(Glutathione peroxidases,GPX)编码基因CsGPX1的功能,本文利用茶树转录组数据克隆获得CsGPX1的编码区全长序列,进行序列比对与分析,在此基础上,将CsGPX1在烟草中进行过表达获得转CsGPX1基因烟草,并比较野生型烟草与转基因烟草耐旱性的差异,验证CsGPX1功能.序列分析表明,CsGPX1编码序列(CDS)长723 bp,编码240个氨基酸.BLAST比对发现,该基因与桃树( Prunus persica)的GPX基因具有高度同源性(>85%). CsGPX1编码的氨基酸序列具有 GPX 蛋白保守特征序列和区别于其他家族成员的特有的结构域——磷脂氢谷胱甘肽过氧化物酶(PHGPX).干旱...

To clarify function of glutathione peroxidase-encoding gene CsGPX1 in Camellia sinensis (L.) O. Kuntze, the full-length sequence of the coding region of CsGPX1 was obtained by transcriptome data of tea plant, and sequence analysis and function prediction were performed. Then, the CsGPX1 was overexpressed in tobacco. Subsequently, the difference in drought tolerance between wild-type and transgenic tobacco was compared to verify the function of CsGPX1. Sequence analysis indicates that CsGPX1 was 723 bp in length, encoding 240 amino acids. The Blast alignment reveals that the GPX showed more tha...