期刊论文

Analysis of the Chloroplast Proteome Difference of ‘Baiye 1' [Camellia sinensis (L.) O Kuntze] during Periodic Albinism

中文

茶叶科学

1000-369X

2019

39

3

325-334

10.3969/j.issn.1000-369X.2019.03.011

国家自然科学基金(31200522) 湖南省教育厅科研项目(15B116) 湖南省财政厅科技专项(湘财教指[2016]175号) 湖南农业大学校青年基金项目(15QN28).

本校为第一机构

白叶1号是一种温度敏感型白化茶树品种,叶绿体的变化是其产生阶段性白化现象的关键因素。本研究以白叶1号鲜叶叶绿体为研究对象,采用双向电泳、质谱鉴定结合生物信息学分析,研究阶段性白化过程中叶绿体蛋白的表达差异,探讨白叶1号阶段性白化现象的分子机制。结果表明,在白叶1号白化前期、白化期和复绿期叶绿体中分别识别726、748、718个蛋白质,其中差异表达的蛋白59个,质谱成功鉴定22个差异表达蛋白。生物信息学分析表明,差异表达蛋白直接或间接参与了光合作用、应激响应、核酸代谢、物质代谢和未知功能等,其中与光合作用相关的差异表达蛋白最多,占31.82%,表明阶段性白化现象可能与这些生理功能...

‘Baiye 1’ is a kind of temperature sensitive tea cultivar. The change of chloroplast is the key factor for the periodic albinism of ‘Baiye 1’. To understand the mechanism of periodic albinism of ‘Baiye 1’, two dimensional electrophoresis (2-DE) and mass spectrometry was adopted to separate and identify the chloroplast proteins, which were significantly changed during the three developmental periods. The results show that 726, 748 and 718 protein spots were separated at the pre-albinistic, albinistic and regreen stages, respectively. The e...