期刊论文、会议论文

Sequencing and Analysis of the Complete Mitochondrial Genome of Penicillium Citrinum in Hunan Yiyang Dark Tea

中文

茶叶科学

1000-369X

2020

40

6

830-844

Editorial Office of Journal of Tea science

1000369X

10.3969/j.issn.1000-369X.2020.06.012

湖南省黑茶金花重点实验室资助项目(湘科规财[2019]8号) 湖南省重点研发计划资助项目(2018NK2036) 安化黑茶非遗传承湖南省社会科学普及基地(湘社普[2019]6号)。

本校为第一机构

对黑茶中分离的一株桔青霉线粒体基因序列进行测定与分析,并探究其与近缘种微生物的系统发育关系。结果表明,桔青霉线粒体基因组是一条全长27 537 bp的环状DNA分子,共编码42个基因(15个蛋白质编码基因,2个rRNA,24个tRNA以及1个独立的ORF),基因组碱基构成为:A(36.17%)、T(37.06%)、C(11.82%)、G(14.95%)。15个蛋白质编码基因均采用典型的ATG作为起始密码子、TAA或TAG作为终止密码子,基因排列顺序与已报道的青霉属物种相似,在进化上较为保守。蛋白质编码基因编码频率较高的氨基酸分别为Leu、Ile、Ser和Phe;RSCU频率最高的4个密码子依次是UUA、AUA、UUU和GGU。24个tRNA基因存在30处G-U错配,均可形成...

In this study, the mitochondrial genome sequence of a strain of Penicillium citrinum isolated from dark tea was determined and analyzed, and its phylogenetic relationship with the closely related microorganisms was explored. The result shows that the mitochondrial genome of Penicillium citrinum is a circular DNA molecule with a length of 27 537 bp, which encodes 42 genes. The genome bases are composed of A (36.17%), T (37.06%), C (11.82%) and G (14.95%). All the 15 protein-coding genes use typical ATG as the start codon, TAA or TAG as the stop ...