期刊论文

中文

茶叶科学

1000-369X

2023

43

3

377-388

10.3969/j.issn.1000-369X.2023.03.008

2022YFE0111200:国家重点研发计划 CARS-19:国家茶叶产业技术体系项目 2021NK1020-3:湖南省产业创新链项目 SCTOF202201:精制川茶四川省重点实验室开放基金

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为了更加高效低成本地制备茶黄素,以儿茶素作为多酚氧化酶蛋白的交联试剂,对巨大芽孢杆菌(Bacillus megaterium)来源的多酚氧化酶进行了酶交联聚集体的制备并应用于茶黄素-3,3’-双没食子酸酯的合成。通过对酶...展开更多 为了更加高效低成本地制备茶黄素,以儿茶素作为多酚氧化酶蛋白的交联试剂,对巨大芽孢杆菌(Bacillus megaterium)来源的多酚氧化酶进行了酶交联聚集体的制备并应用于茶黄素-3,3’-双没食子酸酯的合成。通过对酶交联参数优化以及交联前后的酶催化性能研究,结果表明,在pH 4.0,EGCG为0.5 mg·mL-1,多酚氧化酶活性200 U·mL-1,交联时间50 min时,可以获得交联酶的最佳酶活回收率。与...

In order to prepare theaflavin more efficiently and economically,catechins were used as the cross-linking reagent in this study,and a polyphenol oxidase (tyrosinase) crossed-linked aggregate derived from Bacillus megaterium was prepared and used for the synthesis of the...MORE In order to prepare theaflavin more efficiently and economically,catechins were used as the cross-linking reagent in this study,and a polyphenol oxidase (tyrosinase) crossed-linked aggregate derived from Bacillus megaterium was prepared and used for the synthesis of theaf...