期刊论文

Genetic Diversity Analysis of Standard Variety SSR in French Bean by DUS Testing

中文

分子植物育种

1672-416X

2020

18

18

6197-6204

10.13271/j.mpb.018.006197

湖南省自然科学基金(2017JJ2118)资助。

本校为第一机构

为了对《植物新品种特异性,一致性和稳定性测试指南菜豆》标准品种选用的合理性进行评价,为测试指南进一步的修订和完善提供分子层面的参考依据,本研究对菜豆DUS测试标准品种的遗传多样性进行分析.结果 显示,33对核心引物在26个标准品种中共检测到214个等位变异,平均每对引物检测到了6.49个等位变异,该套引物的Shannon's信息指数在0.515 8～2.125 7之间,均值是1.295 7;多态信息含量(PIC)处于0.248 0～0.860 9之间,平均值为0.604 0.26个标准品种的遗传相似系数在0.03～0.85之间,平均值为0.31,表明品种间遗传多样性较丰富,标准品种选择较为合理.其中仅'双阳大花皮'和'大家花雀蛋间'的遗传相似系数...

In order to evaluate the rationality of the selection of the sample varieties in French bean DUS testing,and provide a molecular basis for revision and improvement the test guide,this study analyzed the genetic diversity of the sample varieties based on the SSR fingerprint database.The results showed that 33 pairs of core primers detected 214 alleles in 26 varieties,and an average of 6.49 alleles were detected for each primer.The Shannon's information index of those primers ranged from 0.515 8 to 2.125 7,with an average of 1.295 7.The polymorphic information content(PIC)ranged from 0.248 0 to ...