期刊论文

Expression Patterns of Genes according to Photosynthesis-related Differential Proteins in High Oleic Acid Rapeseed Isogenic Lines

中文

西南农业学报

1001-4829

2017

30

7

1483-1487

10.16213/j.cnki.scjas.2017.7.003

国家自然科学基金“基于蛋白组学的油菜高油酸关键基因鉴定研究”（31201240）； 国家973计划“主要环境因子及生育期影响油菜油脂积累的分子基础”（2015CB150206）； 国家科技支撑计划“油菜商业化育种研究与模式示范”（2014BAD01B07）； 湖南省科技重大专项“高油酸油菜产业化发展关键技术研究与示范”（2014FJ1006）； 作物种质创新与资源利用国家重点实验室培育基地开放课题“与脂肪酸合成相关基因表达量研究及其功能验证”； 湖南农业大学作物学开放基金“高油酸油菜近等基因系差异基因研究”（ZWKF201302）；

本校为第一机构

【目的】本文探索了光合作用相关基因表达与蛋白表达之间的关系,加速高油酸油菜育种进程。【方法】以高油酸油菜近等基因系出苗后7 d、5~6叶期和越冬期的叶片、花瓣和授粉后20 ~35 d的种子及角果皮为材料,利用定量PCR方法研究9个iTRAQ分析得到的差异蛋白对应基因在不同生育期表达规律。【结果】在出苗后7 d、花期和授粉后20~35 d,高油酸油菜的光合作用能力低于低油酸油菜;5 ~6叶期、越冬期高油酸油菜的光合作用能力高于低油酸油菜。gi1515616基因在高油酸油菜出苗后7 d去根材料及低油酸油菜5 ~6叶期、越冬期叶片和授粉后20 ~35d角果皮中不表达,在花、授粉后20 ~35d种子中均不表达。gil 29785309...

[ Objective] In this paper, the relationship between gene expression and protein expression in photosynthesis were discovered so that the process of high oleic acid rape breeding could be accelerated. [ Method] The 7 days after emergence, 5 - 6 leaf stage, leaves of win- tering period, petals, seeds and pods skins 20 - 35 days after pollination of high oleie acid rapeseed isogenic lines were used as tested mate- rials to find out the expression rules in different development periods. The nine corresponding genes of the differential proteins which derived from the iTRAQ analysis were studied by...