期刊论文

Genetic Diversity Analysis and Identification of Avirulence Genes for Magnaporthe oryzae in Taojiang,Hunan

中文

西南农业学报

1001-4829

2019

32

9

2040-2045

10.16213/j.cnki.scjas.2019.9.012

2016YFD0300700:国家重点研发计划 2014ZX0800102B:中央财政专项 201203014:公益性行业(农业)科研专项 0904:湖南省重点学科建设项目(十二五)

本校为第一机构

【目的】为明确湖南桃江地区稻瘟病菌遗传多样性和无毒基因的组成及变化趋势。【方法】利用8对SSR引物对2017年从湖南桃江病圃多个晚稻品种上分离纯化的29个稻瘟病单孢菌株进行遗传多样性分析,并利用18个已知抗瘟基因的水稻近等基因系(NILs)对其中的19个特异性稻瘟病菌株进行无毒基因鉴定。【结果】在相似系数0.70,差异系数0.30水平下,29个供试菌株可划分为7个宗谱,优势宗谱I有12个菌株,占参测菌数的41.3%,宗谱V有7个菌株,占总菌数的24.1 %,其余5个宗谱分别有1~3个菌株,占总菌数的34.6 %;通过无毒基因鉴定18个菌株致病频率(致病频率=感病水稻数/所有测试水稻品种数×100 %)在35 %~75 %。强致病力...

【Objective】The present paper aimed to clarify the genetic diversity and composition of non-toxic genes of Magnaporthe oryzae in Taojiang.【Method】 Genetic diversity of 29 M. oryzae isolated and purified from several late rice varieties in Taojiang disease nursery was analyzed by 8 pairs of SSR primers. And nontoxic gene of 19 isolates were identified through 18 near-isogeniclines(NILs) whose resistance gene to blast were known.【Result】The results indicated that under the 0.70 similarity coefficient and 0.30 difference coefficient level, th...