【Objective】This paper aimed to explore the effect of different physiological indexes on cold tolerance evaluation under low temperature stress.【Method】With 16 potted potato seedlings as materials,in vitro leaves were subjected to low temperature stress by a program temperature-reducing apparatus to determine the activities of catalase (CAT) active,superoxide dismutase (SOD) active,peroxidase (POD) and content of free proline (PRO),soluble sugar (SS),soluble protein (SP) and malondialdehyde (MDA) in plant leaves.Principal component analysis,...