期刊论文

中文

果树学报

1009-9980

2006

23

4

642-645

10.3969/j.issn.1009-9980.2006.04.036

广东省科技重点计划项目（编号:2003B21601）；

本校为第一机构

柑橘黄龙病（HLB）和衰退病（CTV）是2种危害严重的世界性柑橘病害，国内外对其病原检测的研究相当多。研究建立了同时检测HLB和CTV的多重RT-PCR体系，从影响多重RT-PCR扩增的引物浓度、Mg^2＋浓度、Taq酶浓度、dNTPs浓度、退火温度等方面进行体系优化。结果表明，多重PCR反应的退火温度应优先考虑退火温度高的引物，退火温度高的引物所需浓度要大于相应退火温度低的引物。该体系实现了DNA病原和RNA病原的统一检测．进一步体现了多重RT—PCR的优越性。

Citrus Huanglongbing （HLB） and Citrus tristeza virus （CTV） are two important citrus diseases worldwide. A multiplex reverse transcription-polymerse chain reaction （MuI-RT-PCR） was optimized to simultaneously detect the viruses of HLB and CTV in this article. The reaction of the main ingredients of multiple PCR： Primer,Mg^2＋ ,TaqDNA Polymerase, dNTPs and annealing temperature was examined and optimized. The result showed that the choice of annealing temperature of MuI-RT-PCR should consider the higher annealing-temperature primer and the...