期刊论文

中文

果树学报

1009-9980

2017

34

1

106-114

10.13925/j.cnki.gsxb.20140399

湖南农业大学园艺学开放课题

本校为第一机构

【目的】降低传统化学试剂提取而带来的毒性大风险,试用简便、温和、环保又高效的木瓜蛋白酶法、超滤膜法等分离纯化柿子总多糖提取物(TPsPF)。【方法】以当季成熟‘牛心柿’果实为试材,探讨了木瓜蛋白酶脱蛋白过程中不同处理工艺对TPsPF保留率和蛋白质去除率的影响,并进一步选择MWCO为200/10 ku的超滤膜截留收集分子质量10~200 ku的目标多糖组分,以超滤膜通量和TPsPF截留率为评价指标筛选出超滤工艺条件。【结果】TPsPF木瓜蛋白酶法脱蛋白优化工艺参数:酶用量1.0%,温度60 ℃,处理时间1.5 h,pH值6或7。其中对TPsPF保留率影响较大的因素是处理时间,而对蛋白质去除率影响较大的则是pH值。超滤膜富...

【Objective】TPsPF (total polysaccharides from persimmon fruits) is known to have a variety of pharmacological activities. Extraction of the TPsPF by traditional chemical reagents would potentially have some toxic risk. The extraction of the TPsPF by biological and physical methods would be valuable for the reason of the safety of the extracts. Therefore, papain deproteinization and ultra-filtration membranes were employed for extracting the TPsPF in present study.【Methods】The flesh of persimmon was defated by chloroform and methanol (3∶1) a...