期刊论文

Li, Xin

英文

NATURE PLANTS

2055-026X

2018

4

9

699-710

10.1038/s41477-018-0216-8

We thank Q. Xie (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for sharing the pET28-AtUBA2-His construct, X. Deng (Peking University/ Yale University) for sharing the pGEX-GST-AtUBC8 construct for protein expression, J. Stuttmann (Martin Luther University, Halle-Wittenberg) for sharing the seeds of N.benthaniana eds1 and K. Kirchsteiger and M. Estelle (UCSD) for offering insights in the in vitro ubiquitination assay. Y. Li (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) is thanked for Illumina sequencing data analyses. Y. Qiu (University of British Columbia) is thanked for helping with the phylogenetic analyses of MUSE1. We are grateful to N. Zheng and W. Xing (University of Washington) for enlightening discussions about MUSE1 secondary structure prediction and E3 functions. The Arabidopsis Biological Resource Center is acknowledged for providing the muse1-7 and muse2-1 knockout T-DNA mutant seeds. This work was financially supported by the Natural Sciences and Engineering Research Council (NSERC) Discovery program of Canada and Canadian Foundation for Innovation (CFI) (to X.L.), the Chinese Scholarship Council (to Y.W., Y.H., S.X. and X.C.), the Dewar Memorial fund (to X.L.), NSERC Graduate scholarships (to K.A. and K.C.M.J.) and the UBC Four-Year Fellowship (to F.X., K.C.M.J. and O.X.D.).

本校为其他机构

In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins serve as intracellular immune receptors. Defence signalling by NLRs often requires the formation of NLR heteropairs. Our knowledge of the molecular mechanism regulating this process is limited. In a reverse genetic screen to identify the partner of the Arabidopsis typical NLR, SUPRESSOR OF NPR1, CONSTITUTIVE 1 (SNC1), we discovered three NLRs that are redundantly required for SNC1-mediated defence, which were named SIDEKICK SNC1 1 (SIKIC1), SIKIC2 and SIKIC3. Immunopre...