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Genetic screen identified PRMT5 as a neuroprotection target against cerebral ischemia.

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成果类型:
期刊论文
作者:
Wu, Haoyang;Lv, Peiyuan;Wang, Jinyu;Bennett, Brian;Wang, Jiajia;...
作者机构:
[Wu, Haoyang; Wang, Jinyu; Peng, Yi; Lv, Peiyuan; Lin, Jiaji] Department of Neurology, The Second Affiliated Hospital of Air Force Medical University, Xi'an, China
[Wu, Haoyang; Wang, Jinyu; Lv, Peiyuan; Lin, Jiaji] Basic Medical School, Air Force Medical University, Xi'an, China
[Bennett, Brian] Integrative Bioinformatics Support Group, National Institute of Environmental Health Sciences, Durham, United States
[Wang, Jiajia] Computer Network Information Center, Chinese Academy of Sciences, Beijing, China
[Li, Pishun] College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
语种:
英文
关键词:
cell biology;hippocampal neuronal cell line;mouse;oxygen-glucose deprivation;primary cortical neurons culture;stroke
期刊:
ELIFE
ISSN:
2050-084X
年:
2024
卷:
12
机构署名:
本校为其他机构
院系归属:
动物医学院
摘要:
Epigenetic regulators present novel opportunities for both ischemic stroke research and therapeutic interventions. While previous work has implicated that they may provide neuroprotection by potentially influencing coordinated sets of genes and pathways, most of them remain largely uncharacterized in ischemic conditions. In this study, we used the oxygen-glucose deprivation (OGD) model in the immortalized mouse hippocampal neuronal cell line HT-22 and carried out an RNAi screen on epigenetic regulators. PRMT5 was identified as a novel negative regulator of neuronal cell survival after OGD, whi...

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