期刊论文

中文

中国食品学报

1009-7848

2012

12

3

61-68

10.3969/j.issn.1009-7848.2012.03.010

湖南省自然科学基金项目（07JJ6026）；

本校为第一机构

通过固体平板法,从食堂下水道污泥中筛选到1株耐热脂肪酶产生菌LJM-1,采用摇床培养法对该菌株的产酶条件进行了研究,结果表明,该菌株产脂肪酶的适宜培养基组成是:玉米粉淀粉6.0％、酵母粉3.5％、KH2PO40.3％、MgSO4·7H2O 0.05％、(NH4)2SO4 0.5％、NaCl 0.1％;适宜培养务件:培养温度40℃,初始pH 7.7,摇床转速150 r/min,装液量100 mL/250mL三角瓶,接种量2.5％(体积分数),种龄72 h,发酵时间48h.在此条件下,脂肪酶最高酶活为112.6 U/mL.在单因素试验的基础上,采用正交试验对棉粕发酵脱毒条件进行了优化,得到最佳发酵条件是:初始pH 7.7,培养温度40℃,接种量1.5％,发酵周期2d,酶活力达131.6 U/mL.

A thermostable lipase producing strain LJM-1 was screened from canteen sewage sludge by solid plate method.The fementation conditions for lipase production were studied by using the methods of percussion table. The re- suits showed that the optimized culture conditions were as follows：corn starch 6.0%, yeast powder 3.5%, KH2PO 40.3%, MgSO4·7H2O 0.05%, （NH4）2SO4 0.5%, NaC1 0.1%, cultural temperature 40℃, the initial pH value 7.7, rotatory speed 150 r/min, 100 mL liquid medium in 250 mL erlemneyer flask, the vaccination quantity 2.5%（V/V）,...