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柱色谱分离纯化五倍子没食子酸

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成果类型:
期刊论文
作者:
廖格;卲元元;熊硕;邓娣;肖文军
通讯作者:
Xiao, Wenjun
作者机构:
[卲元元; 熊硕; 邓娣; 肖文军; 廖格] National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Provincial Co-Innovation Center of Ultilization of Botanical Function Ingredients, Hunan Agricultural University, Changsha, China
[廖格] College of Horticulture and Landscape, Hainan University, Haikou, China
[卲元元; 熊硕; 邓娣; 肖文军; 廖格] Botanical Resource Engineering Department of College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China
通讯机构:
National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Provincial Co-Innovation Center of Ultilization of Botanical Function Ingredients, Hunan Agricultural University, Changsha, China
语种:
中文
关键词:
五倍子;没食子酸;柱色谱;大孔吸附树脂;分离纯化
关键词(英文):
Column chromatography;Galla chinensis;Gallic acid;Macroporous resin;Separation and purification
期刊:
中国食品学报
ISSN:
1009-7848
年:
2015
卷:
15
期:
7
页码:
131-138
基金类别:
“十二五”国家科技支撑计划项目(2011BAD10B00) 国家农业科技成果转化资金项目(2011GB2D200010)
机构署名:
本校为第一且通讯机构
院系归属:
园艺园林学院
摘要:
目的:优化筛选分离纯化五倍子没食子酸的最优树脂及其工艺参数。方法:以五倍子水提取液为原料,比较NKA-2、LS303、HZ816、HP-20、XAD-16、HPD700、HPD500、S-8、AB-8、D_(10)1、D301 11种树脂对五倍子没食子酸的静态吸附与解吸效果,筛选最优树脂。采用单因素试验、正交试验与验证性试验,优化最优树脂动态吸附与解吸五倍子没食子酸的工艺参数。结果:NKA-2树脂为最优树脂,静态吸附量与解吸率分别达到94.856 mg/g和89.64%;当主要考虑没食子酸纯度时,其最优动态吸附与解吸工艺参数是:上样质量浓度7 mg/mL,上样体积7BV,上样流速2 BV/h,洗脱剂乙醇体积分数70%,洗脱体积2.5 BV,洗脱流速3 BV/h,所获五倍...
摘要(英文):
Objective: Studies on separation and purification of gallic acid from Galla chinensis by different resins. Methods: The research compared the absorption capacity and desorbtion effect with different concentration of alcohol of 11 kinds of resins: NKA-2, LS303, HZ816, HP-20, XAD-16, HPD700, HPD500, S-8, AB-8, D101, D301, and the best processing parameters were optimized based on the adsorption and desorption results. Results: NKA-2 was the best one. An optimum purification parameter with sample concentration 7 mg/mL, sample volume 7 BV, flow rate 2 BV/h, elution ethanol concentration 70%, eluti...

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